fixes #16500

modules/python/package/template/config.py.in

+import os
+
 BINARIES_PATHS = [
     @CMAKE_PYTHON_BINARIES_PATH@
 ] + BINARIES_PATHS

modules/python/python_loader.cmake

@@ -58,7 +58,13 @@ if(NOT OpenCV_FOUND)  # Ignore "standalone" builds of Python bindings
     else()
       list(APPEND CMAKE_PYTHON_BINARIES_INSTALL_PATH "os.path.join(${CMAKE_PYTHON_EXTENSION_INSTALL_PATH_BASE}, '${OPENCV_LIB_INSTALL_PATH}')")
     endif()
-    string(REPLACE ";" ",\n    " CMAKE_PYTHON_BINARIES_PATH "${CMAKE_PYTHON_BINARIES_INSTALL_PATH}")
+    set(CMAKE_PYTHON_BINARIES_PATH "${CMAKE_PYTHON_BINARIES_INSTALL_PATH}")
+    if (WIN32 AND HAVE_CUDA)
+      if (DEFINED CUDA_TOOLKIT_ROOT_DIR)
+        list(APPEND CMAKE_PYTHON_BINARIES_PATH "os.path.join(os.getenv('CUDA_PATH', '${CUDA_TOOLKIT_ROOT_DIR}'), 'bin')")
+      endif()
+    endif()
+    string(REPLACE ";" ",\n    " CMAKE_PYTHON_BINARIES_PATH "${CMAKE_PYTHON_BINARIES_PATH}")
     configure_file("${PYTHON_SOURCE_DIR}/package/template/config.py.in" "${__python_loader_install_tmp_path}/cv2/config.py" @ONLY)
     install(FILES "${__python_loader_install_tmp_path}/cv2/config.py" DESTINATION "${OPENCV_PYTHON_INSTALL_PATH}/cv2/" COMPONENT python)
   endif()